{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312554/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312554"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Aquaporin-1 sustains lymphangiogenic responses in inflammatory microenvironments","description":"Intestinal lymphatic vessels are essential for dietary lipid absorption and immune cell trafficking. Specialized villus lymphatic capillaries, lacteals, undergo constant VEGF-C-dependent renewal to maintain their function in a high-osmolarity, inflammatory microenvironment exposed to dietary by-products. The mechanisms of lacteal adaptation remain incompletely understood. We integrated new and published single-cell RNA-sequencing data to profile murine small-intestinal lymphatic endothelial cells (LECs) and identified three distinct subsets. Lacteal LECs display a transcriptional signature resembling Ptx3⁺ immune-interacting LECs characterized by high expression of Aqp1, encoding the aquaporin-1 water channel. LEC-specific deletion of Aqp1 reduced lacteal length, impaired lipid uptake, and limited weight gain on a high-fat diet, underscoring the importance of water homeostasis in lacteal maintenance. Mechanistically, AQP1 promoted LEC migration under osmotic stress via β-catenin mediated actin remodeling. At the transcriptional level, AQP1 partially rescued or alleviated the transcriptional program induced by osmotic stress in LECs. AQP1 was also upregulated during inflammatory remodeling in secondary lymphedema and lymphatic malformations, but not during embryonic lymphangiogenesis. These findings link lacteal regeneration to inflammatory lymphatic remodeling and highlight tissue osmolarity as a key biophysical factor in postnatal lymphangiogenesis.","dates":{"publication":"2026/05/13"},"accession":"GSE312554","cross_references":{"GSM":["GSM9348304","GSM9348305","GSM9348298","GSM9348299","GSM9348302","GSM9348303","GSM9348300","GSM9348301"],"GPL":["34295"],"GSE":["312554"],"taxon":["Homo sapiens"]}}