GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312606/primary200OKTranscriptomicsHomo sapiens OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312606GEOGSEfalseEnrichment of CD4-CD8- cytotoxic Vδ1/3 T cells in pulmonary tuberculosis diseaseTuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a leading cause of global morbidity and mortality. Although gamma-delta (γδ) T cells have increasingly been suggested to contribute to the TB immune response, quantitative and qualitative differences in this immune cell compartment between healthy and diseased individuals are not well-characterized. In this study, we used single-cell RNA sequencing to provide a high-resolution characterization of CD4-CD8- γδ T cells in peripheral blood across healthy Mtb-(non-)sensitized and TB disease pre-/post-treatment cohorts. We found an activated and cytotoxic gene signature in γδ T cells of TB disease compared to both healthy cohorts. Strikingly, these differences persisted through one year following TB disease diagnosis. These transcriptomic differences were largely mediated by an NK-like cytotoxic Vδ1 and Vδ3 subset that was enriched in TB disease. Our findings suggest long-lasting changes in the CD4-CD8- γδ T cell compartment and highlight specific subsets as potentially important in TB.2026/08/14GSE312606GSM9350002GSM9350003GSM9350004GSM9350005GSM9350000GSM9350001GSM9349978GSM9349979GSM9349998GSM9349976GSM9349999GSM9349977GSM9349992GSM9349993GSM9349990GSM9349991GSM9349996GSM9349975GSM9349997GSM9349994GSM9349995GSM9349989GSM9349987GSM9349988GSM9350006GSM9350007GSM9349981GSM9349982GSM9349980GSM9349985GSM9349986GSM9349983GSM934998424676312606Homo sapiens[42662901]