GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313027/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313027GEOGSEfalseTranscriptomics Identifies Mild Hyperthermia-Conditioned Tumor Supernatant as an Inducer of HepG2 MaturationThis study aimed to explore whether tumor supernatant (TSN) under mild hyperthermia induces the transformation of HepG2 cells into mature hepatocyte-like cells and to identify the underlying mechanisms involved in this process. HepG2 cells were treated at a mild hyperthermic temperature of 42.5°C for 60 minutes, and the resulting supernatant was collected and labeled as 42.5TSN. As a control, HepG2 cells were also treated at a normal temperature of 37°C for 60 minutes, and the supernatant was collected and labeled as 37TSN. The collected TSN was then concentrated and applied to untreated normal HepG2 cells to detect differential gene expression.2026/07/01GSE313027GSM9359359GSM9359360GSM9359355GSM9359356GSM9359357GSM935935824676313027Homo sapiens[42358519]