{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313040/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Fundulus heteroclitus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313040"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"New insights into SREB orphan receptor roles in the ovary, using comparative transcriptomics across 3 fishes","description":"Mummichog (Fundulus heteroclitus) ovary in vitro assays were completed using methods modified from Rajeswari et al., 2020. Briefly, female F. heteroclitus (n = 20) were euthanized in 300 mg/L buffered MS-222 (Syncaine®, tricaine methanesulfonate, Syndel, Ferndale, WA, USA), weighed, and measured (total length (mm), TL). Ovaries from each fish were partitioned into 5 sections of approximately equal size. Each of four of these sections were immersed into an assigned well within a 24-well plate containing 990 μl of culture media (90% Leibovitz’s L-15 medium [Sigma Cat# L1518], 1% penicillin-streptomycin [Gibco™ Cat# 15070-063], 0.5% bovine serum albumin [Sigma Cat# 81053N]), with either a dimethyl sulfoxide (DMSO) control or one of three potential agonist treatments, 8535 (5 μM), PT-91 (5 μM), or PNX-20 (100 nM). Agonists were dissolved in DMSO and added to their respective wells in 10μl aliquots to achieve desired final concentrations in a final volume of 1ml in each well. The fifth section of ovary was photographed on a Sedgewick rafter cell with a Jenoptik ProgRes® C5 microscope camera to characterize egg stages for each fish. Four plates containing n=4-6 replicates per treatment were incubated for 6 hours at 28 ℃ and each plate represented a temporal replicate. After incubation, ovarian tissues were snap frozen in RNAlater™ (Invitrogen Cat# AM7020) for transcriptome analysis and culture media were stored in glass tubes at -80°C for further steroid quantification analysis via liquid chromatography tandem mass spectrometry (LC-MS/MS).","dates":{"publication":"2026/08/17"},"accession":"GSE313040","cross_references":{"GSM":["GSM9359623","GSM9359624","GSM9359613","GSM9359625","GSM9359614","GSM9359615","GSM9359626","GSM9359627","GSM9359616","GSM9359628","GSM9359617","GSM9359618","GSM9359619","GSM9359620","GSM9359621","GSM9359622"],"GPL":["34850"],"GSE":["313040"],"taxon":["Fundulus heteroclitus"],"PMID":["[42563402]"]}}