{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313095/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Genome binding/occupancy profiling by high throughput sequencing","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313095"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Circadian entrainment of pancreatic islet function and of the pancreatic islet transcriptome is regulated by glucocorticoid signaling [Multiome]","description":"Glucocorticoid (GC) hormone secretion follows a clear circadian pattern and regulates diverse physiological and molecular functions, including glucose homeostasis. The function of GCs is mediated by the glucocorticoid receptor (GR), a ligand-induced nuclear hormone receptor that promotes expression of genes in a cell-type-specific manner. However, the GR cistrome and the corresponding regulatory role of GCs on pancreatic β-cells remain poorly understood. To address these knowledge gaps, we used a combination of transcriptional, epigenetic, and physiological assays to systematically delineate the role of GC signaling in β-cells. Our studies provide the first description of the β-cell GR cistrome demonstrating its enrichment for transcriptional regulators of insulin secretion, β-cell identity, and circadian rhythms. In addition, we also find that GR-dependent β-cell enhancers display circadian expression patterns in vivo and their circadian expression can be amplified and/or phase-shifted by timed administration of synthetic GC in mice. Finally, we also show that restoration of GC signaling in circadian-disrupted mice enhances diurnal glucose tolerance, β-cell function, and circadian rhythmicity of genes enriched for pathways controlling cellular circadian rhythms and insulin secretion. Our findings suggest that the circadian timing of glucocorticoid secretion plays an important role in modulating β-cell circadian gene expression, function and the regulation of glucose tolerance. These findings have direct implications for understanding the physiological role of GCs in the regulation of diurnal glucose homeostasis, and for developing pharmacological approaches using GC therapy.","dates":{"publication":"2026/09/28"},"accession":"GSE313095","cross_references":{"GSM":["GSM9360830","GSM9360831","GSM9360825","GSM9360826","GSM9360824","GSM9360829","GSM9360827","GSM9360828"],"GPL":["24247"],"GSE":["313095"],"taxon":["Mus musculus"]}}