GEOTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313127GEOGSEfalsePharmacological activation of UPR pathways and its impact on gene expressionThis study investigates how pharmacologic activationn of the PERK branch of the unfolded protein response (UPR) enhances the immunoregulatory function of FoxP3⁺ regulatory T cells (Tregs) in the context of intestinal inflammation. Using ER stress-inducing agents and PERK activators, combined with flow cytometry, bulk RNA sequencing, in vitro Treg conversion and suppression assays, ELISA, qRT-PCR, and immunoblotting, we demonstrate that PERK activation reduces gut inflammation and promotes Treg-mediated immune regulation. These findings identify the PERK pathway as a novel immunologic target for enhancing Treg function and mitigating chronic intestinal inflammation, offering translational relevance for therapeutic strategies in inflammatory bowel disease (IBD).2026/05/31GSE313127GSM9362383GSM9362382GSM9362387GSM9362386GSM9362385GSM936238434290313127Mus musculus