{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313502/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["synthetic construct"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313502"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Direct RNA sequencing of in vitro transcribed mRNA modified with 5-methoxyuracil","description":"The aim of this study was to understand potential biases in the incorporation of modified nucleotides, specifically 5-methoxyuracil, in in vitro transcription reactions by different T7 polymerases through Nanopore Direct RNA Sequencing. Incorporation by a range of T7 polymerases using different DNA templates was sequenced using RNA002 and RNA004 flow cell chemistry. Analysis was performed using basecall error-based modification detection (EpiNano).","dates":{"publication":"2026/08/20"},"accession":"GSE313502","cross_references":{"GSM":["GSM9369095","GSM9369096","GSM9369093","GSM9369094","GSM9369091","GSM9369092","GSM9369090","GSM9369103","GSM9369104","GSM9369101","GSM9369102","GSM9369099","GSM9369089","GSM9369100","GSM9369097","GSM9369098"],"GPL":["25738"],"GSE":["313502"],"taxon":["synthetic construct"]}}