GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313502/primaryOK200Othersynthetic constructOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313502GEOGSEfalseDirect RNA sequencing of in vitro transcribed mRNA modified with 5-methoxyuracilThe aim of this study was to understand potential biases in the incorporation of modified nucleotides, specifically 5-methoxyuracil, in in vitro transcription reactions by different T7 polymerases through Nanopore Direct RNA Sequencing. Incorporation by a range of T7 polymerases using different DNA templates was sequenced using RNA002 and RNA004 flow cell chemistry. Analysis was performed using basecall error-based modification detection (EpiNano).2026/08/20GSE313502GSM9369095GSM9369096GSM9369093GSM9369094GSM9369091GSM9369092GSM9369090GSM9369103GSM9369104GSM9369101GSM9369102GSM9369099GSM9369089GSM9369100GSM9369097GSM936909825738313502synthetic construct