GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313515/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313515GEOGSEfalseDysregulated Cholesterol Crystal-STING Pathway in Microglia Drives Neuroinflammation after Ischemic StrokeDysregulated lipid metabolism is increasingly implicated in microglial activation, yet the specific consequences of massive cholesterol overload following permanent ischemia remain elusive. Using single-cell RNA sequencing in permanent distal middle cerebral artery occlusion (dMCAO) and perioperative ischemic stroke (PIS) models, we elucidated the pathogenic role of intracellular cholesterol crystals. We identified a progressive accumulation of cholesterol crystals within peri-infarct microglia during the subacute phase, driven by a specific downregulation of the lipid-processing enzyme lipoprotein lipase (LPL).2026/08/19GSE313515GSM9369471GSM936947228330313515Mus musculus[42578421]