{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Non-coding RNA profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313744"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The MIME-seq technique allows to monitor the interaction of small non-coding RNAs with Argonaute proteins and their transfer to other cells","description":"The MIME-seq2.0 technique, developed by Mandlbauer et al., utilizes a chimeric HEN1 methyltransferase engineered to bind to Argonaute proteins (MIME enzyme) to protect the terminal sugar of small non-coding RNAs (sncRNAs) from oxidation, a reaction that inhibits RNA ligation and cloning. In this study, we confirm that the MIME-seq technique efficiently distinguish oxidized and non-oxidized sncRNAs using RNA sequencing and quantitative PCR. Upon transfection of the insulin-secreting cell line MIN6B1 with or without the MIME enzyme, we observed a significant loss in the detection of miRNAs following RNA oxidation, with a strong recovery rate in MIME-expressing cells. Comparison of the sncRNA profile in wild type and MIME-expressing cells demonstrated protection from oxidation not only of miRNAs, but also of some Y-RNA and tRNA-derived fragments. Immunoprecipitation with Ago2 antibodies confirmed that the protected Y-RNA and tRNA fragments bind to Argonaute proteins. We also used this system to track miRNA transfer between cells via extracellular vesicles (EVs). miRNAs released in EVs of MIME-expressing Jurkat T cells or C2C12 myotubes and delivered to MIN6B1 cells were protected from oxidation, enabling sensitive and specific detection of the transferred miRNAs in the receiving cells. Overall, the MIME-seq technique provides a powerful tool for analyzing RNA methylation and Argonaute binding in living cells and offers new possibilities for tracking RNA transfer across different cell types.","dates":{"publication":"2026/05/12"},"accession":"GSE313744","cross_references":{"GSM":["GSM9373406","GSM9373409","GSM9373407","GSM9373408","GSM9373412","GSM9373413","GSM9373410","GSM9373411"],"GPL":["34475"],"GSE":["313744"],"taxon":["Mus musculus"],"PMID":["[41844788]"]}}