GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313857/primaryOK200TranscriptomicsNothobranchius furzeriExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313857GEOGSEfalseEffect of Alendronate on Survival and Bone Properties in Nothobranchius furzeri: Insights from a Model of Accelerated AgingAlendronate, a nitrogen containing bisphosphonate, effectively inhibits osteoclastic bone resorption and is considered as first line treatment of osteoporosis and Paget´s disease. Additionally, several studies suggest a beneficial effect on mortality that goes beyond life-extending effects attributed to the well-established fracture risk reduction. However, mechanisms of the mortality reducing effect of bisphosphonates are unclear. The turquoise killifish Nothobranchius furzeri (N.furzeri), a well-known model of accelerated aging, exhibits an extremely short life cycle and thus could represent a suitable model for survival studies. The aim of this study was to test the effect of alendronate on the life span and bone properties of N.furzeri. 89 female fish were divided at the age of 16 weeks into a treatment (10µM alendronate, n=44) and a control (n=45) group and monitored for survival. Vertebral bodies of deceased fish were examined by micro-CT and bone histomorphometry. RNA sequencing and gene expression analysis was done from isolated tissues of heart, spleen, liver, muscle and kidney. Comparing both groups, the alendronate group showed a significantly higher survival (p=0.0070). Micro-CT evaluation showed significantly higher values for bone (+2%; p=0.0013) and tissue mineral density (+3%; p=0.0159) in the control group. Bone histomorphometry displayed significant higher values for number of osteoblasts/bone perimeter (p=0.0340) and number of osteoblasts (p=0.0335) in the alendronate group. RNA sequencing revealed differences in both gene expression and biological function with alendronate treatment. Looking at the gene expression levels, we determined a reduced ZNF703 expression in the alendronate group. Our results indicate that alendronate has a positive effect on the survival of N.furzeri and alters bone mineralization. Furthermore, it effects several non-bone related pathways.2026/06/01GSE313857GSM9376698GSM9376731GSM9376730GSM9376713GSM9376735GSM9376712GSM9376734GSM9376733GSM9376711GSM9376699GSM9376710GSM9376732GSM9376706GSM9376728GSM9376705GSM9376727GSM9376704GSM9376726GSM9376725GSM9376703GSM9376709GSM9376708GSM9376707GSM9376729GSM9376720GSM9376702GSM9376724GSM9376701GSM9376723GSM9376722GSM9376700GSM9376721GSM9376739GSM9376717GSM9376716GSM9376738GSM9376715GSM9376737GSM9376736GSM9376714GSM9376719GSM937671836277313857Nothobranchius furzeri