{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313973/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313973"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Endothelial Ext1 Is Essential for Glycocalyx Integrity and Inflammatory Resolution in Acute Lung Injury","description":"The endothelial glycocalyx (eGCX) heparan sulfate (HS) is essential for vascular barrier function and inflammation regulation. To elucidate the endothelial cell-autonomous role of the HS synthase Ext1, we generated endothelial-specific Ext1-deficient mice (Ext1flox/flox; VE-cadherin–Cre) and performed endotoxin challenge experiments. Transcriptomic analysis of pulmonary endothelial cells revealed marked down-regulation of HS- and glycocalyx-related genes and up-regulation of immune activation pathways. Fluorescence imaging and dextran extravasation assays confirmed significant depletion of lectin- and HS-labeled glycocalyx and barrier dysfunction. Electron microscopy demonstrated severe baseline loss of luminal glycocalyx. Following LPS administration, Ext1-deficient mice exhibited altered cytokine kinetics, delayed clearance of neutrophils and CD11b⁺ myeloid cells, increased late-phase macrophage accumulation, and prolonged pulmonary edema, indicating impaired resolution of inflammation. Gene set enrichment analysis revealed up-regulation of leukocyte transendothelial migration, chemokine signaling, and VEGF pathways, consistent with sustained endothelial activation.","dates":{"publication":"2026/10/07"},"accession":"GSE313973","cross_references":{"GSM":["GSM9378682","GSM9378683","GSM9378684","GSM9378685","GSM9378681","GSM9378686","GSM9378687","GSM9378688"],"GPL":["21163"],"GSE":["313973"],"taxon":["Mus musculus"],"PMID":["[42820257]"]}}