{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314018/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314018"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Muco-Obstructive Lung Disease Exhibits Distinct Hypoxic and Inflammatory Airway Epithelial Gene Pathways","description":"Intraluminal obstruction produces chronic airway epithelial hypoxia. We investigated how chronic airway epithelial hypoxia and inflammatory stimuli (supernatatants of mucopurulent materials: SMM) interact in human bronchial epithelial (HBE) cultures and resected bronchiectatic lungs to produce the diseased epithelial environment characteristic of muco-obstructive diseases. Results: Hypoxia and supernatants of mucopurulent materials (SMM) regulated largely separate transcriptional programmes regardless of treatment order. PTGS2 and CXCL8 represented a small number of genes additively upregulated by both hypoxia and SMM. HIF1A knockdown and EPAS1 knockdown produced distinct, often opposing, effects on hypoxia-responsive pathways. HIF1A dominated upregulation of glycolytic pathway signalling. EPAS1 regulated genes that increase mucus concentration under hypoxia (e.g., SCNN1G). Conclusion: Chronic hypoxia and inflammation regulated distinct genes/pathways that combine to produce complex diseased environments in muco-obstructed airways. HIF1α and EPAS1 regulated distinct hypoxia-induced transcriptional responses.","dates":{"publication":"2026/08/17"},"accession":"GSE314018","cross_references":{"GSM":["GSM9379961","GSM9379960","GSM9379967","GSM9379966","GSM9379969","GSM9379968","GSM9745176","GSM9379963","GSM9745177","GSM9379962","GSM9745178","GSM9379965","GSM9745179","GSM9379964","GSM9745173","GSM9745174","GSM9745175","GSM9379959","GSM9379992","GSM9379991","GSM9379994","GSM9379993","GSM9379990","GSM9379956","GSM9379999","GSM9379955","GSM9379958","GSM9379957","GSM9379996","GSM9745187","GSM9379952","GSM9379995","GSM9745188","GSM9379951","GSM9379998","GSM9379954","GSM9745189","GSM9379953","GSM9379997","GSM9745183","GSM9745184","GSM9745185","GSM9745186","GSM9745180","GSM9745181","GSM9745182","GSM9380018","GSM9380016","GSM9380017","GSM9380014","GSM9380015","GSM9380012","GSM9379981","GSM9380013","GSM9379980","GSM9380010","GSM9379983","GSM9379982","GSM9380011","GSM9379989","GSM9379988","GSM9379985","GSM9379984","GSM9379987","GSM9379986","GSM9745190","GSM9745191","GSM9745192","GSM9380009","GSM9380007","GSM9380008","GSM9380005","GSM9380006","GSM9380003","GSM9380004","GSM9379970","GSM9380001","GSM9380002","GSM9379972","GSM9380000","GSM9379971","GSM9379978","GSM9379977","GSM9379979","GSM9379974","GSM9379973","GSM9379976","GSM9379975"],"GPL":["24676"],"GSE":["314018"],"taxon":["Homo sapiens"]}}