{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314073/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314073"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of KRAS mutants in a panel of isogenic human pancreatic ductal adenocarcinoma (PDAC) cell lines","description":"KRAS is mutated more than 90% of pancreatic ductal adenocarcinomas (PDAC), where hotspot alterations in codons 12, 13, and 61 drive tumor initiation and progression. Although distinct biochemical properties have been reported for individual KRAS mutants, a comprehensive characterization of allele-specific differences in PDAC cells remains unresolved. Here, we systematically interrogated the molecular consequences of seven common KRAS mutant variants by reconstituting 4 isogenic, KRAS-deficient PDAC cell lines and performing bulk RNA-sequencing. We found that baseline cellular state, rather than allele identity, was the predominant driver of molecular variation across all samples. Pathway analyses highlighted that KRAS mutants upregulated inflammatory and immune-related pathways, including TNFα signaling via NFκB, IL2-STAT5 signaling, and epithelial–mesenchymal transition, while downregulating interferon responses and hypoxia-associated. Importantly, no robust allele-specific molecular programs were identified across all samples. Our study establishes a comprehensive resource for investigating mutant KRAS transcriptome in PDAC and demonstrates that cellular context exerts a stronger influence than allele identity in shaping molecular profiles.","dates":{"publication":"2026/09/10"},"accession":"GSE314073","cross_references":{"GSM":["GSM9381146","GSM9381168","GSM9381167","GSM9381145","GSM9381144","GSM9381166","GSM9381143","GSM9381165","GSM9381164","GSM9381142","GSM9381141","GSM9381163","GSM9381140","GSM9381162","GSM9381161","GSM9381160","GSM9381139","GSM9381138","GSM9381137","GSM9381159","GSM9381158","GSM9381157","GSM9381156","GSM9381155","GSM9381176","GSM9381154","GSM9381153","GSM9381175","GSM9381152","GSM9381174","GSM9381173","GSM9381151","GSM9381150","GSM9381172","GSM9381171","GSM9381170","GSM9381149","GSM9381148","GSM9381147","GSM9381169"],"GPL":["34284"],"GSE":["314073"],"taxon":["Homo sapiens"],"PMID":["[42720273]"]}}