GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314073/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314073GEOGSEfalseTranscriptomic profiling of KRAS mutants in a panel of isogenic human pancreatic ductal adenocarcinoma (PDAC) cell linesKRAS is mutated more than 90% of pancreatic ductal adenocarcinomas (PDAC), where hotspot alterations in codons 12, 13, and 61 drive tumor initiation and progression. Although distinct biochemical properties have been reported for individual KRAS mutants, a comprehensive characterization of allele-specific differences in PDAC cells remains unresolved. Here, we systematically interrogated the molecular consequences of seven common KRAS mutant variants by reconstituting 4 isogenic, KRAS-deficient PDAC cell lines and performing bulk RNA-sequencing. We found that baseline cellular state, rather than allele identity, was the predominant driver of molecular variation across all samples. Pathway analyses highlighted that KRAS mutants upregulated inflammatory and immune-related pathways, including TNFα signaling via NFκB, IL2-STAT5 signaling, and epithelial–mesenchymal transition, while downregulating interferon responses and hypoxia-associated. Importantly, no robust allele-specific molecular programs were identified across all samples. Our study establishes a comprehensive resource for investigating mutant KRAS transcriptome in PDAC and demonstrates that cellular context exerts a stronger influence than allele identity in shaping molecular profiles.2026/09/10GSE314073GSM9381146GSM9381168GSM9381167GSM9381145GSM9381144GSM9381166GSM9381143GSM9381165GSM9381164GSM9381142GSM9381141GSM9381163GSM9381162GSM9381140GSM9381161GSM9381160GSM9381139GSM9381138GSM9381137GSM9381159GSM9381158GSM9381157GSM9381156GSM9381155GSM9381176GSM9381154GSM9381153GSM9381175GSM9381152GSM9381174GSM9381173GSM9381151GSM9381150GSM9381172GSM9381171GSM9381170GSM9381149GSM9381148GSM9381147GSM938116934284314073Homo sapiens[42720273]