{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314271/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314271"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq analysis of PAPD5 inhibitor effects in expanded human induced pluripotent stem cell-derived cardiomyocytes","description":"Human induced pluripotent stem cells (hiPSCs) were differentiated into cardiomyocytes using a conventional monolayer protocol to generate non-expanded cardiomyocytes (N-CMs). A subset of cardiomyocytes was further replated and cultured in the presence of CHIR99021 to generate expanded cardiomyocytes (E-CMs). During the expansion phase, E-CMs were treated with the PAPD5 inhibitor BCH001 (E-CM-B) or RG7834 (E-CM-R) to prevent cellular senescence. RNA sequencing was performed to characterize transcriptional differences associated with senescence and mitochondrial maturation. Treatment with BCH001 during cardiomyocyte expansion attenuated the expression of senescence-associated markers and promoted gene expression profiles consistent with mitochondrial maturation. This dataset provides transcriptomic insights into the molecular effects of PAPD5 inhibition on expanded hiPSC-derived cardiomyocytes.","dates":{"publication":"2026/06/11"},"accession":"GSE314271","cross_references":{"GSM":["GSM9391987","GSM9391997","GSM9391986","GSM9391989","GSM9391988","GSM9391994","GSM9391993","GSM9391996","GSM9391985","GSM9391995","GSM9391990","GSM9391992","GSM9391991"],"GPL":["24676"],"GSE":["314271"],"taxon":["Homo sapiens"],"PMID":["[42271510]"]}}