GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314282/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314282GEOGSEfalseDevelopmental senescence orchestrates hyaloid vessel regression in the postnatal eyeThe mammalian eye develops in concert with coordinated growth and remodeling of three vascular networks: the transient hyaloid vasculature, the choroid and retinal plexus. While retinal and choroidal systems support visual function in the mature eye, the hyaloid network plays a vital yet temporary role supporting the developing lens and inner retina. Its postnatal regression is essential for optical clarity, yet the mechanisms guiding the process remain incompletely understood. Here, single-cell RNA sequencing of postnatal mouse hyaloids reveals pervasive cellular senescence, characterized by cell cycle arrest and a distinctive secretory profile within hyaloid vascular smooth muscle, endothelial, and immune cell populations, displaying high Cdkn1a and expression of SASP factors. Genetic ablation of Cdkn1a impedes normal hyaloid regression, demonstrating that developmental senescence is essential for timely vascular remodeling and functions alongside apoptosis and macrophage-mediated clearance. These findings identify a previously unrecognized senescence-driven mechanism orchestrating hyaloid vessel involution during early ocular development, broadening the understanding of vascular remodeling in the eye.2026/09/10GSE314282GSM9392302GSM939230119057314282Mus musculus