GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314400/primaryOK200TranscriptomicsHomo sapiens OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314400GEOGSEfalseCIPHER-seq enables low-stress intracellular multimodal profiling of immune activationThis study introduces CIPHER-seq, an optimized intracellular CITE-seq workflow that minimizes fixation-induced cellular stress while enabling simultaneous measurement of intracellular proteins and transcriptomes in the same cell. Peripheral blood mononuclear cells (PBMCs) were processed using either CIPHER-seq or a commercial intracellular protocol (Proteintech) under unstimulated or PMA/ionomycin-stimulated conditions. Single-cell RNA and antibody-derived tag (ADT) libraries were generated using 10x Genomics Flex chemistry. The dataset enables benchmarking of intracellular chemistries, assessment of RNA–protein concordance, and characterization of cytokine-driven immune activation at single-cell resolution.2026/04/08GSE314400GSM9395707GSM9395706GSM9395705GSM939570434281314400Homo sapiens