GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_T.pyriformis.RNAseq.exon.featurecounts.bed.gzftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_T.pyriformis.ATACseq.RPKM.bigwigftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.12hpm.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.log.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.3hpm.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.1.5hpm.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_Tp.MC.log.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.Refeed.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_HiC.tt.log.fa.dsg.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.8hpm.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_HiC.tt.log.fa.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.star20.combined.mcoolftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314518/suppl/GSE314518_MC.tt.24hpm.combined.mcoolprimary200OKTranscriptomics Tetrahymena thermophilaTetrahymena pyriformis Genome binding/occupancy profiling by high throughput sequencing OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314518GEOGSEfalseChromatin ends and promoter loops organize chromosome-sized domains in TetrahymenaThree-dimensional genome architecture shapes how regulatory elements controls gene expression, yet how compact unicellular genomes are folded is poorly understood. Ciliates such as Tetrahymena thermophila carry a fragmented, gene-dense somatic macronucleus (MAC) and a silent germline micronucleus (MIC), imposing distinct constraints on chromosome folding. Here we combine nucleosome-resolution Micro-C, ATAC-seq and RNA-seq to map 3D chromatin organization across Tetrahymena life cycle stages and in the related ciliate Tetrahymena pyriformis. We find that MAC chromosomes lack strong mammalian-style compartments and TADs but behave as short interaction units whose telomere-capped ends form accessible, strongly interacting hubs. Within these units, chromatin loops concentrate at promoter-proximal open chromatin and are closely associated with transcription level. During conjugation, long-range internal loops and promoter–promoter contacts are transiently weakened and then rebuilt, while end–end interactions persist. Similar promoter- and end-anchored folding in T. pyriformis points to a conserved ciliate strategy for organizing gene-rich genomes in three dimensions.2026/07/02GSE314518GSM9400230GSM9400212GSM9400211GSM9400210GSM9400216GSM9400215GSM9400214GSM9400213GSM9400219GSM9400218GSM9400217GSM9400223GSM9400222GSM9400221GSM9400220GSM9400205GSM9400227GSM9400204GSM9400226GSM9400225GSM9400224GSM9400209GSM9400208GSM9400229GSM9400207GSM9400206GSM94002283644435705314518 Tetrahymena thermophilaTetrahymena pyriformis