GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314639/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314639GEOGSEfalseOxygenated perfusion enhances hepatocyte function in human iPSC-liver tissueThis study aimed to create functional and scalable tissue from hiPSC-derived liver organoids (hiPSC-LOs) by establishing an oxygenation system utilizing a decellularized liver (DL), which retains the parenchymal and vascular extracellular matrix to support cell adhesion and medium perfusion. An oxygenation system for hiPSC-LO-engrafted DLs (hiPSC-LT) was established using perfusion of oxygen-enriched medium containing artificial red blood cells. Perfusion with oxygenated medium containing artificial red blood cells suppressed cell death and promoted hepatic function of hiPSC-LTs by mimicking the physiological oxygen concentration found in the fetal liver. The oxygenation system using DLs and artificial red blood cells effectively supported the generation of transplantable, functional hiPSC-LT.2026/06/23GSE314639GSM9407377GSM9407378GSM9407375GSM9407376GSM9407373GSM9407374GSM940737217303314639Homo sapiens