GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314814/primaryOK200TranscriptomicsDrosophila melanogasterExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314814GEOGSEfalseMyc sustains Regional and Sex-biased Organ Zonation in the Drosophila IntestineOrgans exhibit distinct transcriptional and metabolic territories; how and why such organ zonation differs between the sexes is unclear. Using adult Drosophila intestines, we combine whole-organ 3D cellular mapping with metabolic profiling to uncover regional differences in enterocyte size and metabolism that vary by sex and genotype. Zonation arises from opposing, region-specific and sex-biased actions of the sex determinant Doublesex (Dsx) and the transcription factor Myc. In enterocytes, male-specific Dsx suppresses Myc, rendering its expression female-biased. Dsx–Myc antagonism enlarges female enterocytes, increasing their ploidy in specific gut regions. It also drives a female-specific lipid profile, marked by sex-biased expression of a cluster of lipases and selective triacylglycerol storage in the anterior midgut. Myc activity in female enterocytes sustains peripheral lipid reserves and fecundity. These findings identify relevant roles for Myc in sex differentiation and the intestine as a key, previously overlooked contributor to sex differences in lipid metabolism.2026/04/30GSE314814GSM9411120GSM9411122GSM9411111GSM9411121GSM9411110GSM9411113GSM9411112GSM9411115GSM9411114GSM9411117GSM9411116GSM9411108GSM9411119GSM9411107GSM9411118GSM941110917275314814Drosophila melanogaster