GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314869/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314869GEOGSEfalsePotential of an in vitro mouse testicular organ culture system as a platform for mechanism-based evaluation of testicular toxicityThis study examined testicular toxicity induced by methoxyacetic acid (MAA) using an in vitro mouse testicular organ culture system. Testis tissues were cultured for 4 weeks and then exposed to MAA (1–4 mM) for 24 hours. Higher MAA concentrations reduced germ-cell viability and spermatocyte populations, accompanied by increased apoptosis. RNA-seq analysis revealed early transcriptional perturbations at 1 mM and broad suppression of cell-cycle and meiotic pathways at 4 mM. These findings characterize dose-dependent molecular and cellular changes associated with MAA-induced testicular toxicity.2026/05/12GSE314869GSM9414772GSM9414771GSM9414770GSM9414776GSM9414765GSM9414775GSM9414764GSM9414763GSM9414773GSM9414762GSM9414769GSM9414779GSM9414768GSM9414778GSM9414767GSM9414766GSM941477734290314869Mus musculus[41903202]