GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315070/primaryOK200TranscriptomicsEscherichia coliExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315070GEOGSEfalseComparison of global mRNA decay at single-gene resolution in E. coli Δrnb Δrnr versus E. coli Δrnb Δrnr ΔfbpWe determined whether deletion of the glycolysis gene fbp broadly modulates transcript stability in a strain where PNPase is the sole exoribonuclease. We analyzed global RNA degradation at single-gene resolution after rifampicin treatment of E. coli Δrnb Δrnr Δfbp versus the isogenic parental strain, E. coli Δrnb Δrnr, using RNA sequencing. ERCC spike-in standards were incorporated before cDNA library preparation and used for normalization of read-counts-per-gene. We found 1321 transcripts with significantly different degradation patterns between the strains.2026/07/16GSE315070GSM9420779GSM9420778GSM9420777GSM9420799GSM9420776GSM9420798GSM9420775GSM9420797GSM9420774GSM9420796GSM9420795GSM9420773GSM9420772GSM9420794GSM9420771GSM9420793GSM9420792GSM9420770GSM9420791GSM9420790GSM9420769GSM9420768GSM9420789GSM9420767GSM9420766GSM9420788GSM9420765GSM9420787GSM9420786GSM9420764GSM9420763GSM9420785GSM9420762GSM9420784GSM9420783GSM9420761GSM9420782GSM9420760GSM9420781GSM942078034685315070Escherichia coli