GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315071/primaryOK200TranscriptomicsEscherichia coliExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315071GEOGSEfalseHigh-throughput sequencing of RNA isolated by crosslinking immunoprecipitation with CsdA or PNPase in E.coliOur results show the RNA transcripts in complex with the helicase CsdA or the exoribonuclease PNPase. We used CsdA or a catalytically inactive variant of PNPase (PNPase D492G) as bait to capture enriched substrates bound by the enzymes. Sequencing of RNA isolated by crosslinking immunoprecipitation with these enzymes identified numerous transcripts bound to CsdA or PNPase.2026/07/16GSE315071GSM9420801GSM9420800GSM9420808GSM9420807GSM9420806GSM9420805GSM9420804GSM9420803GSM942080234685315071Escherichia coli