GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315144/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315144GEOGSEfalseArtificial intelligence-guided single-cell transcriptomic analysis for identifying O-GlcNAc transferase in fibroblast-like synoviocytes as a therapeutic target for rheumatoid arthritisRheumatoid arthritis (RA) is a chronic autoimmune disease characterized by systemic inflammation, synovial hyperplasia, and the destruction of bone and cartilage. Despite the use of various immunosuppressive disease-modifying antirheumatic drugs (DMARDs), a considerable proportion of RA patients remain symptomatic. As RA progresses, fibroblast-like synoviocytes (FLSs) undergo a phenotypic transition to an aggressive state, making them attractive non-immune cellular targets for RA treatment. However, there are currently no clinically available therapies that selectively ablate RA-FLSs due to the lack of specific molecular targets. Our research focuses on the knockdown of the OGT and SAP130 genes in RA-FLSs. OGT and SAP130 are expressed in the cell of RA-FLSs and plays a crucial role in their aggressive transition. By specific knockout of OGT and SAP130 we observed a significant supression of aggressive transformations of RA-FLS.2026/09/01GSE315144GSM9422110GSM9422111GSM9422112GSM9422113GSM9422114GSM9422115GSM9422116GSM9422106GSM9422117GSM9422118GSM9422107GSM9422108GSM9422119GSM942210918460315144Homo sapiens