GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315176/primary200OKTranscriptomicsDrosophila melanogasterExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315176GEOGSEfalseLifelong consumption of freeze-dried Parabacteroides distasonis improves gut barrier integrity and extends lifespan and health span in Drosophila melanogasterChronic inflammation has been shown to be associated with aging. In Drosophila melanogaster, age-related inflammation in the gut results in a disruption of gut barrier integrity, which can lead to reduced intestinal function and death. The anaerobic bacterium Parabacteroides distasonis (Pd) has anti-inflammatory properties and can attenuate tumorigenesis and promote intestinal barrier integrity in a mouse model of colorectal cancer. Here, we show that administration of Pd to adult Drosophila significantly increases mean lifespan and promotes maintenance of climbing ability throughout adult life. Furthermore, it enhances gut barrier integrity in aged adults. RNA-Seq analysis of Pd treated adult guts indicates that the enhanced healthspan is accompanied by transcriptional upregulation of genes involved in DNA replication and maintenance of genome integrity. These results suggest that Pd can induce a transcriptional shift towards augmented DNA repair in aging gut that prevents the breakdown of gut barrier integrity and promotes healthy aging.2026/06/23GSE315176GSM9422660GSM9422650GSM9422661GSM9422662GSM9422651GSM9422663GSM9422652GSM9422664GSM9422653GSM9422654GSM9422665GSM9422666GSM9422655GSM9422656GSM9422657GSM9422658GSM9422659GSM942264934457315176Drosophila melanogaster