GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315511/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315511GEOGSEfalseAttenuation of oxygen-induced neovascularization and inflammation by neutralizing antibody to VEGFA and/or ANG-2Neovascularization is a major cause of blindness in various retinal diseases, and inflammation aggravates the pathological and clinical conditions of these diseases. VEGFA or ANG-2 neutralizing antibodies have been used to block pathological neovascularization. In this work, the effects of intraocular administration of neutralizing antibodies against VEGFA, ANG-2, or bispecific to these two factors on pathological findings were examined in the oxygen-induced retinopathy (OIR) mouse model. At both postnatal day (P)17 and P19, anti-VEGFA and -ANG-2 administration suppressed neovascularization, and the bispecific antibody attenuated neovascularization more efficiently. However, oxygen-induced vaso-obliteration was not modified by these antibodies. Numbers of photoreceptor, amacrine, and bipolar cells were reduced in the OIR retina, and the antibodies reversed these changes. Microglia-specific gene expression increased in the OIR retina, and administration of the antibodies reduced the IBA1-positive area in the OIR retina, although these antibodies did not affect marker gene expression. Labeled VEGFA and ANG-2 were found to be co-localized with microglia, suggesting that VEGFA and ANG-2 affect microglia activation directly. Taken together, neutralizing antibody to VEGFA or ANG-2 attenuated oxygen-induced neovascularization and inflammation, and the bispecific antibody more efficiently suppressed some features than the single antibody to VEGFA or ANG-2.2026/04/15GSE315511GSM9429790GSM9429794GSM9429793GSM9429792GSM9429791GSM9429798GSM9429797GSM9429796GSM9429795GSM9429813GSM9429812GSM9429811GSM9429799GSM9429810GSM9429817GSM9429816GSM9429815GSM9429814GSM9429819GSM9429818GSM9429787GSM9429820GSM9429786GSM9429785GSM9429802GSM9429824GSM9429801GSM9429823GSM9429822GSM9429789GSM9429800GSM9429788GSM9429821GSM9429806GSM9429805GSM9429804GSM9429803GSM9429809GSM9429808GSM942980724247315511Mus musculus[41944819]