GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315534/suppl/filelist.txtftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315534/suppl/GSE315534_RAW.tarftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315534/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315534GEOGSEfalseSingle-cell RNA sequencing of primary colorectal cancer, matched adjacent normal tissues, and paired liver metastasesThis study will perform single-cell RNA sequencing on primary tumor tissues (T) and matched adjacent non-tumor tissues (N) from six colorectal cancer patients, along with paired liver metastatic lesions (M) from three of these patients, to construct an integrated single-cell atlas spanning primary tumors, adjacent normal tissues, and distant metastases. This multi-region, patient-matched design enables systematic dissection of cellular heterogeneity, dynamic immune responses, and key molecular mechanisms driving metastasis in colorectal cancer.2026/04/02GSE315534GSM9430358GSM9430357GSM9430359GSM9430365GSM9430354GSM9430353GSM9430364GSM9430356GSM9430355GSM9430366GSM9430361GSM9430360GSM9430352GSM9430363GSM943036224676315534Homo sapiens