{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315654/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Oryza sativa"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315654"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Integrated Multi-omics Analysis Reveals that Hybrid Rice Reprograms Carbon Metabolism to Confer Drought Resistance","description":"Drought severely limits global rice production, and exploiting heterosis offers a promising route to improve drought resistance. However, the molecular mechanisms underlying drought resistant heterosis remain largely unknown. Here, we integrated multi-omics analyses of the flag leaves of elite hybrid rice Hanyou73 (HY73) and its parental lines, Hanhui3 (HH3) and Huhan7B (HH7B), during grain filling under drought. We uncover a non-additive effect (NAE)-driving carbon metabolic reprogramming fundamental to HY73’s superior performance under drought. Proteomic and metabolomic profiling revealed that HY73 preferentially channels photosynthetic carbon into a hybrid-specific monosaccharides–acetyl-CoA–lipid (MOS–AcCoA–Lipid) shunt, effectively diverting carbon into fatty acyls (FA) and prenol lipids (PR) that serve as vital energy reserves, membrane stabilizers, and precursors for hormone biosynthesis. Importantly, NAE-driving AcCoA accumulation correlates with elevated lysine acetylation (Kac) in HY73, establishing a bridge between metabolic flux and post-translational regulation. Acetylomic analyses pinpointed acetylation at lysine 155 (K155ac) on OsPGM3, a previously uncharacterized cytosolic phosphoglucomutase, as a pivotal regulatory switch. CRISPR/Cas9-mediated gene editing and biochemical assays demonstrated that drought-induced deacetylation activates OsPGM3, ensuring sufficient glucose-6-phosphate (G6P) for the lipid shunt while maintaining stable glucose-1-phosphate (G1P)-mediated sucrose export vital for yield potential. Thus, K155ac precisely fine-tunes OsPGM3 activity, acting as a post-translational checkpoint responsive to drought-induced carbon shifts. Together, our findings establish a hybrid-specific NAE–carbon flux–Kac regulatory axis that precisely balances drought adaptation with yield maintenance, offering new targets for breeding climate-resilient hybrid rice.","dates":{"publication":"2026/05/18"},"accession":"GSE315654","cross_references":{"GSM":["GSM9434062","GSM9434051","GSM9434050","GSM9434061","GSM9434060","GSM9434049","GSM9434059","GSM9434048","GSM9434047","GSM9434058","GSM9434057","GSM9434046","GSM9434045","GSM9434056","GSM9434055","GSM9434054","GSM9434053","GSM9434052"],"GPL":["35184"],"GSE":["315654"],"taxon":["Oryza sativa"]}}