{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315794/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Methylation profiling"],"species":["Homo sapiens"],"gds_type":["Methylation profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315794"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Non-ablative fractional laser 1940nm treatment series modulates epigenetic pathways and signs of skin aging: a split face investigation","description":"Skin aging is driven by cumulative environmental damage and epigenetic dysregulation, yet it is unclear whether energy-based rejuvenation therapies can durably remodel the cutaneous methylome. To address this, we conducted a split-face, paired longitudinal study in 22 adults treated with a 1940-nm non-ablative fractional laser (NAFL). Epidermal samples were collected by tape stripping from treated and contralateral control sites at baseline, immediately after the first treatment, and at 1, 3, and 6 months. Enzymatic methyl-sequencing profiled ~3.8 million CpGs per sample; clinical endpoints included pigmentation, texture, and global aesthetic scores. No significant differentially methylated regions (DMRs) were detected immediately post-treatment, but 635 DMRs emerged from 1 month after the final session, expanding through 3 months and stabilizing by 6 months. These loci were enriched for pathways involved in epidermal differentiation, collagen organization, wound response, and stem-cell maintenance, and showed selective resetting at Polycomb-regulated and WNT-signaling genes. NAFL reversed age-associated methylation change at >83% of age-linked CpGs and engaged gene sets previously implicated in molecular rejuvenation. Treatment also induced transient, treatment-specific DMRs related to immune and stress responses. Epigenetic remodeling paralleled significant clinical improvements, indicating that 1940-nm NAFL drives durable molecular rejuvenation rather than transient repair.","dates":{"publication":"2026/07/08"},"accession":"GSE315794","cross_references":{"GSM":["GSM9437495","GSM9437496","GSM9437497","GSM9437530","GSM9437531","GSM9437498","GSM9437499","GSM9437510","GSM9437532","GSM9437511","GSM9437533","GSM9437534","GSM9437512","GSM9437513","GSM9437535","GSM9437514","GSM9437536","GSM9437537","GSM9437515","GSM9437516","GSM9437517","GSM9437518","GSM9437519","GSM9437493","GSM9437494","GSM9437520","GSM9437521","GSM9437500","GSM9437522","GSM9437523","GSM9437501","GSM9437502","GSM9437524","GSM9437503","GSM9437525","GSM9437504","GSM9437526","GSM9437505","GSM9437527","GSM9437528","GSM9437506","GSM9437507","GSM9437529","GSM9437508","GSM9437509"],"GPL":["34281"],"GSE":["315794"],"taxon":["Homo sapiens"],"PMID":["[42380171]"]}}