GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315794/primaryOK200Methylation profilingHomo sapiensMethylation profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315794GEOGSEfalseNon-ablative fractional laser 1940nm treatment series modulates epigenetic pathways and signs of skin aging: a split face investigationSkin aging is driven by cumulative environmental damage and epigenetic dysregulation, yet it is unclear whether energy-based rejuvenation therapies can durably remodel the cutaneous methylome. To address this, we conducted a split-face, paired longitudinal study in 22 adults treated with a 1940-nm non-ablative fractional laser (NAFL). Epidermal samples were collected by tape stripping from treated and contralateral control sites at baseline, immediately after the first treatment, and at 1, 3, and 6 months. Enzymatic methyl-sequencing profiled ~3.8 million CpGs per sample; clinical endpoints included pigmentation, texture, and global aesthetic scores. No significant differentially methylated regions (DMRs) were detected immediately post-treatment, but 635 DMRs emerged from 1 month after the final session, expanding through 3 months and stabilizing by 6 months. These loci were enriched for pathways involved in epidermal differentiation, collagen organization, wound response, and stem-cell maintenance, and showed selective resetting at Polycomb-regulated and WNT-signaling genes. NAFL reversed age-associated methylation change at >83% of age-linked CpGs and engaged gene sets previously implicated in molecular rejuvenation. Treatment also induced transient, treatment-specific DMRs related to immune and stress responses. Epigenetic remodeling paralleled significant clinical improvements, indicating that 1940-nm NAFL drives durable molecular rejuvenation rather than transient repair.2026/07/08GSE315794GSM9437495GSM9437496GSM9437497GSM9437530GSM9437531GSM9437498GSM9437499GSM9437510GSM9437532GSM9437511GSM9437533GSM9437534GSM9437512GSM9437513GSM9437535GSM9437514GSM9437536GSM9437537GSM9437515GSM9437516GSM9437517GSM9437518GSM9437519GSM9437493GSM9437494GSM9437520GSM9437521GSM9437500GSM9437522GSM9437523GSM9437501GSM9437502GSM9437524GSM9437503GSM9437525GSM9437504GSM9437526GSM9437505GSM9437527GSM9437528GSM9437506GSM9437507GSM9437529GSM9437508GSM943750934281315794Homo sapiens[42380171]