GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315822/primary200OKGenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315822GEOGSEfalseChIP-seq analysis of human CENP-A nucleosomes following CENP-A overexpressionCentromeres ensure accurate chromosome segregation, and their identity is specified epigenetically by the histone H3 variant CENP-A. We combined cryogenic electron microscopy with chromatin immunoprecipitation (ChIP-CryoEM) to determine the structures of human CENP-A nucleosomes isolated directly from cells. Sequencing analysis of the CENP-A nucleosome samples revealed that, upon CENP-A overexpression, CENP-A nucleosomes are enriched on centromeric α-satellite DNA and are also ectopically distributed along chromosome arms.2026/08/28GSE315822GSM9437933GSM94379342279024676315822Homo sapiens