{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315928/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315928"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Differential T cell clonal dynamics underlie outcomes to frontline chemoimmunotherapy in advanced gastric cancer [paired scRNA/TCR-seq]","description":"The addition of aPD1 to 5-FU/platinum in advanced gastric cancer (GC) yields variable responses. To understand cooperativity between chemotherapy and immunotherapy, we previously reported a phase II trial sequentially adding pembrolizumab to 5-FU/platinum. In this study, we use single-cell RNA- and TCR-sequencing to analyze 66,813 T cells from primary tumor biopsies pre-treatment, post-chemotherapy, and post-immunotherapy in 33 patients. We observed greater abundance, persistence, and recruitment of T cells with predicted tumor-reactivity in patients with prolonged progression-free survival (slow progressors). Increased B cell abundance and predicted B cell to T cell interactions supported T cell memory and co-stimulation, providing a mechanism for increased abundance and persistence of progenitor-exhausted and tumor-reactive T cells in slow progressors. T cell clones emerging in the tumor after immunotherapy were in the blood before treatment only in slow progressors. Our study thus highlights pre-treatment and early chemotherapy-induced T cell dynamics and B cell to T cell interactions that may drive durable response to chemoimmunotherapy in GC.","dates":{"publication":"2026/04/01"},"accession":"GSE315928","cross_references":{"GSM":["GSM9440223","GSM9440189","GSM9440222","GSM9440188","GSM9440221","GSM9440220","GSM9440187","GSM9440227","GSM9440226","GSM9440225","GSM9440224","GSM9440182","GSM9440181","GSM9440180","GSM9440186","GSM9440185","GSM9440262","GSM9440261","GSM9440184","GSM9440183","GSM9440260","GSM9440219","GSM9440218","GSM9440217","GSM9440212","GSM9440256","GSM9440179","GSM9440255","GSM9440211","GSM9440178","GSM9440254","GSM9440177","GSM9440210","GSM9440176","GSM9440253","GSM9440216","GSM9440215","GSM9440259","GSM9440214","GSM9440258","GSM9440257","GSM9440213","GSM9440171","GSM9440170","GSM9440252","GSM9440175","GSM9440251","GSM9440174","GSM9440173","GSM9440250","GSM9440172","GSM9440209","GSM9440208","GSM9440207","GSM9440206","GSM9440245","GSM9440201","GSM9440200","GSM9440244","GSM9440243","GSM9440242","GSM9440249","GSM9440205","GSM9440248","GSM9440204","GSM9440203","GSM9440247","GSM9440246","GSM9440169","GSM9440202","GSM9440241","GSM9440240","GSM9440239","GSM9440234","GSM9440233","GSM9440232","GSM9440199","GSM9440231","GSM9440198","GSM9440238","GSM9440237","GSM9440236","GSM9440235","GSM9440193","GSM9440192","GSM9440191","GSM9440190","GSM9440197","GSM9440230","GSM9440196","GSM9440195","GSM9440194","GSM9440229","GSM9440228"],"GPL":["24676"],"GSE":["315928"],"taxon":["Homo sapiens"]}}