{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315929/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315929"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Differential T cell clonal dynamics underlie outcomes to frontline chemoimmunotherapy in advanced gastric cancer [TCR-seq]","description":"The addition of aPD1 to 5-FU/platinum in advanced gastric cancer (GC) yields variable responses. To understand cooperativity between chemotherapy and immunotherapy, we previously reported a phase II trial sequentially adding pembrolizumab to 5-FU/platinum. In this study, we use single-cell RNA- and TCR-sequencing to analyze 66,813 T cells from primary tumor biopsies pre-treatment, post-chemotherapy, and post-immunotherapy in 33 patients. We observed greater abundance, persistence, and recruitment of T cells with predicted tumor-reactivity in patients with prolonged progression-free survival (slow progressors). Increased B cell abundance and predicted B cell to T cell interactions supported T cell memory and co-stimulation, providing a mechanism for increased abundance and persistence of progenitor-exhausted and tumor-reactive T cells in slow progressors. T cell clones emerging in the tumor after immunotherapy were in the blood before treatment only in slow progressors. Our study thus highlights pre-treatment and early chemotherapy-induced T cell dynamics and B cell to T cell interactions that may drive durable response to chemoimmunotherapy in GC.","dates":{"publication":"2026/04/01"},"accession":"GSE315929","cross_references":{"GSM":["GSM9440267","GSM9440388","GSM9440387","GSM9440266","GSM9440386","GSM9440265","GSM9440385","GSM9440264","GSM9440269","GSM9440389","GSM9440268","GSM9440380","GSM9440384","GSM9440263","GSM9440383","GSM9440382","GSM9440381","GSM9440498","GSM9440377","GSM9440376","GSM9440497","GSM9440496","GSM9440375","GSM9440495","GSM9440374","GSM9440379","GSM9440499","GSM9440378","GSM9440490","GSM9440373","GSM9440494","GSM9440493","GSM9440372","GSM9440492","GSM9440371","GSM9440491","GSM9440370","GSM9440289","GSM9440288","GSM9440287","GSM9440286","GSM9440281","GSM9440280","GSM9440285","GSM9440284","GSM9440283","GSM9440282","GSM9440399","GSM9440278","GSM9440398","GSM9440277","GSM9440276","GSM9440397","GSM9440396","GSM9440275","GSM9440279","GSM9440270","GSM9440391","GSM9440390","GSM9440395","GSM9440274","GSM9440273","GSM9440394","GSM9440393","GSM9440272","GSM9440392","GSM9440271","GSM9440502","GSM9440501","GSM9440500","GSM9440299","GSM9440298","GSM9440297","GSM9440292","GSM9440291","GSM9440290","GSM9440296","GSM9440295","GSM9440294","GSM9440293","GSM9440520","GSM9440524","GSM9440403","GSM9440402","GSM9440523","GSM9440522","GSM9440401","GSM9440521","GSM9440400","GSM9440517","GSM9440516","GSM9440515","GSM9440514","GSM9440519","GSM9440518","GSM9440513","GSM9440512","GSM9440511","GSM9440510","GSM9440506","GSM9440505","GSM9440504","GSM9440503","GSM9440509","GSM9440508","GSM9440507","GSM9440421","GSM9440300","GSM9440420","GSM9440425","GSM9440304","GSM9440424","GSM9440303","GSM9440302","GSM9440423","GSM9440422","GSM9440301","GSM9440418","GSM9440417","GSM9440416","GSM9440415","GSM9440419","GSM9440410","GSM9440530","GSM9440414","GSM9440413","GSM9440412","GSM9440411","GSM9440528","GSM9440407","GSM9440527","GSM9440406","GSM9440405","GSM9440526","GSM9440525","GSM9440404","GSM9440409","GSM9440408","GSM9440529","GSM9440322","GSM9440443","GSM9440442","GSM9440321","GSM9440441","GSM9440320","GSM9440440","GSM9440447","GSM9440326","GSM9440325","GSM9440446","GSM9440445","GSM9440324","GSM9440444","GSM9440323","GSM9440319","GSM9440439","GSM9440318","GSM9440438","GSM9440317","GSM9440316","GSM9440437","GSM9440432","GSM9440311","GSM9440310","GSM9440431","GSM9440430","GSM9440436","GSM9440315","GSM9440435","GSM9440314","GSM9440313","GSM9440434","GSM9440433","GSM9440312","GSM9440429","GSM9440308","GSM9440428","GSM9440307","GSM9440427","GSM9440306","GSM9440426","GSM9440305","GSM9440309","GSM9440465","GSM9440344","GSM9440464","GSM9440343","GSM9440342","GSM9440463","GSM9440462","GSM9440341","GSM9440469","GSM9440348","GSM9440468","GSM9440347","GSM9440467","GSM9440346","GSM9440345","GSM9440466","GSM9440461","GSM9440340","GSM9440460","GSM9440339","GSM9440459","GSM9440338","GSM9440333","GSM9440454","GSM9440453","GSM9440332","GSM9440452","GSM9440331","GSM9440330","GSM9440451","GSM9440458","GSM9440337","GSM9440336","GSM9440457","GSM9440456","GSM9440335","GSM9440455","GSM9440334","GSM9440450","GSM9440329","GSM9440449","GSM9440328","GSM9440448","GSM9440327","GSM9440487","GSM9440366","GSM9440365","GSM9440486","GSM9440485","GSM9440364","GSM9440484","GSM9440363","GSM9440369","GSM9440489","GSM9440368","GSM9440488","GSM9440367","GSM9440362","GSM9440483","GSM9440482","GSM9440361","GSM9440481","GSM9440360","GSM9440480","GSM9440476","GSM9440355","GSM9440475","GSM9440354","GSM9440353","GSM9440474","GSM9440473","GSM9440352","GSM9440359","GSM9440479","GSM9440358","GSM9440478","GSM9440357","GSM9440356","GSM9440477","GSM9440472","GSM9440351","GSM9440471","GSM9440350","GSM9440470","GSM9440349"],"GPL":["15520"],"GSE":["315929"],"taxon":["Homo sapiens"]}}