GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316006/primaryOK200GenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316006GEOGSEfalseTCR Signaling Induced Kdm6b Antagonizes Ezh2 to Sustain Tissue Regulatory T Cells [ATAC-seq]Regulatory T cells (Tregs) expressing Forkhead Box P3 (Foxp3) play crucial roles in maintaining immune tolerance and tissue integrity. Ezh2, a Histone H3 lysine 27 (H3K27) methyltransferase, is known as a crucial regulator of Treg cell identity and suppressive function upon activation, raising a question about whether overactivation of this pathway would further boost Treg cell function. Here, we demonstrate that lysine demethylase Kdm6b catalyzing the reverse reaction to Ezh2 is also required for Treg cell identity and function after activation. Treg-specific deletion of Kdm6b impairs tissue Treg cell fate and function, such as in the lung after injury or adipose tissue during animal aging. Mechanistically, T cell receptor (TCR) signaling upregulates Kdm6b expression and recruits it to Foxp3-bound chromatin to restrict H3K27me3. This antagonism helps suppress the expression of inflammatory cytokines and promote the expression of Treg functional genes, thus supporting tissue Treg cells.2026/04/08GSE316006GSM9442930GSM9442931GSM9442932GSM9442933GSM9442927GSM9442928GSM9442929GSM9442923GSM9442934GSM9442924GSM9442925GSM944292634328316006Mus musculus