{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316007/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Rattus norvegicus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316007"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell sequencing and spatial transcriptomics revealed that the coacervate–cell–drug system effectively protects articular cartilage in osteoarthritic rats from wear and degenerative deterioration.","description":"We employed DNBSEQ technology to perform single-cell RNA sequencing of rat macrophages, as well as single-cell and spatial transcriptomic analyses of rat knee joint tissues. Single-cell profiling of macrophages demonstrated that the coacervate–cell–drug system effectively reversed the pro-inflammatory phenotype by driving the transition of M1 macrophages toward a reparative M2c phenotype. Single-cell analysis of joint tissues further revealed strong interactions between exogenous GFP-labeled stem cells delivered by the coacervate–cell–drug system and endogenous joint-resident cells. Spatial transcriptomic analysis demonstrated that the coacervate–cell–drug system effectively promotes extracellular matrix repair in osteoarthritic cartilage.","dates":{"publication":"2026/06/27"},"accession":"GSE316007","cross_references":{"GSM":["GSM9442963","GSM9442941","GSM9442942","GSM9442964","GSM9442943","GSM9442944","GSM9442960","GSM9442961","GSM9442940","GSM9442962","GSM9442938","GSM9442939","GSM9442956","GSM9442935","GSM9442957","GSM9442958","GSM9442936","GSM9442937","GSM9442959","GSM9442952","GSM9442953","GSM9442954","GSM9442955","GSM9442950","GSM9442951","GSM9442949","GSM9442945","GSM9442946","GSM9442947","GSM9442948"],"GPL":["31008"],"GSE":["316007"],"taxon":["Rattus norvegicus"]}}