GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316007/primaryOK200TranscriptomicsRattus norvegicus OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316007GEOGSEfalseSingle-cell sequencing and spatial transcriptomics revealed that the coacervate–cell–drug system effectively protects articular cartilage in osteoarthritic rats from wear and degenerative deterioration.We employed DNBSEQ technology to perform single-cell RNA sequencing of rat macrophages, as well as single-cell and spatial transcriptomic analyses of rat knee joint tissues. Single-cell profiling of macrophages demonstrated that the coacervate–cell–drug system effectively reversed the pro-inflammatory phenotype by driving the transition of M1 macrophages toward a reparative M2c phenotype. Single-cell analysis of joint tissues further revealed strong interactions between exogenous GFP-labeled stem cells delivered by the coacervate–cell–drug system and endogenous joint-resident cells. Spatial transcriptomic analysis demonstrated that the coacervate–cell–drug system effectively promotes extracellular matrix repair in osteoarthritic cartilage.2026/06/27GSE316007GSM9442963GSM9442941GSM9442942GSM9442964GSM9442943GSM9442944GSM9442960GSM9442961GSM9442940GSM9442962GSM9442938GSM9442939GSM9442956GSM9442935GSM9442957GSM9442958GSM9442936GSM9442937GSM9442959GSM9442952GSM9442953GSM9442954GSM9442955GSM9442950GSM9442951GSM9442949GSM9442945GSM9442946GSM9442947GSM944294831008316007Rattus norvegicus