GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316051/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316051GEOGSEfalseTo screen the epigentic facters' effect on mESC state transition, Naïve state, Primed state.The transcriptional and epigenetic status restricts the self-renewal ability and lineage specificity of the naïve and primed mouse embryonic stem cells (mESC). CRISPR/Cas9-based functional screening coupled with single cell RNA-seq (CROP-seq) establishes relationships between gRNA-mediated knockout genotypes and transcriptome phenotypes, and is a novel tool to unravel gene regulatory networks. Here, we used CROP-seq approach to dissect the transcriptional and epigenetic regulation of the pluripotency network in mESCs.2026/08/19GSE316051GSM9443799GSM9443800GSM9443795GSM9443796GSM9443797GSM9443798GSM9443793GSM944379424247316051Mus musculus[42462715]