{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316313/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316313"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing of MC38 subcutaneous allograft tumors in C57BL/6J mice treated with FOLFOX alone or in combination with a composite probiotics","description":"This study employs single-cell RNA sequencing (scRNA-seq) to investigate the impact of a probiotic consortium, alone or in combination with the chemotherapeutic regimen FOLFOX, on the tumor microenvironment (TME) of MC38 colorectal adenocarcinoma allografts in C57BL/6J mice. MC38 tumor-bearing mice were divided into three experimental groups: untreated control, FOLFOX-treated, and FOLFOX plus composite probiotics-treated. Tumors were harvested at a defined endpoint, dissociated into single-cell suspensions, and subjected to scRNA-seq using the 10x Genomics platform. The resulting data enable high-resolution characterization of transcriptional changes across immune, stromal, and malignant cell populations within the TME. This dataset aims to elucidate how combinatorial probiotic and chemotherapy intervention reshapes cellular composition, cell-state transitions, and intercellular communication networks, potentially revealing mechanisms of enhanced anti-tumor immunity or therapy resistance.","dates":{"publication":"2026/06/01"},"accession":"GSE316313","cross_references":{"GSM":["GSM9449362","GSM9449361","GSM9449360","GSM9449359","GSM9449358","GSM9449357","GSM9449356","GSM9449355","GSM9449354"],"GPL":["24247"],"GSE":["316313"],"taxon":["Mus musculus"]}}