{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316332/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Rattus norvegicus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316332"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Nano-layered Tendon Patch to Orchestrate Timely Interactions between Tendon Stem/Progenitor Cells and Macrophages","description":"Tendon regeneration remains limited by the unmet challenge of precisely controlling the intensity and duration of local inflammation, often resulting in scarred healing. Here, we present a novel, micro-thin, nano-layered patch that employs Layer-by-Layer (LbL) self-assembly for the precise controlled release of small molecules, Oxo-M and 4-PPBP, to address this technical challenge. This novel combination of small molecules exhibits distinct functions in modulating the polarization of macrophages and the tenogenic differentiation of tendon stem/progenitor cells (TSCs). The customized, temporal release of these small molecules effectively modulated the critical crosstalk between TSCs and macrophages, thereby promoting regenerative tendon healing. The nano-LbL tendon patch successfully mitigated early inflammation, promoted matrix synthesis, and regulated later tissue remodeling in vivo. Robust scRNA-seq and CellChat analysis delineated the temporal-orchestrated communication between cell types across healing phases, confirming the critical interplays between TSCs, tenocytes, and macrophages. Our nano-layered LbL nanopatch represents a highly translational approach for achieving scarless tendon regeneration. In addition, this study elucidated the essential signaling pathways regulating TSC-macrophage crosstalk, advancing our understanding of tendon biology and pathology.","dates":{"publication":"2026/09/10"},"accession":"GSE316332","cross_references":{"GSM":["GSM9449671","GSM9449669","GSM9449668","GSM9449672"],"GPL":["25947"],"GSE":["316332"],"taxon":["Rattus norvegicus"],"PMID":["[42664711]"]}}