{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316540/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316540"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"α2,6-Sialoglycan-regulated anti-tumor immunity of tumor-infiltrating CD8+ T cells in the tumor microenvironment","description":"Cell-surface sialoglycans overexpressed on cancer cells suppress the activation of tumor-infiltrating immune cells (TIICs), and have emerged as a promising target for cancer immunotherapy. However, the functional roles of sialylation in TIICs remain incompletely understood. Here, we develop the single-cell correlative analysis of α2,3- and α2,6-linked sialoglycans and transcriptomes (scCAST_3&6) strategy to correlate cell-surface sialylation in a linkage-specific manner with gene expression of the same cells by single-cell RNA sequencing. Applying scCAST_3&6, we discover that α2,3- and α2,6-sialoglycans are distinctively regulated in specific TIIC subpopulations. Importantly, downregulated α2,6-sialoglycan is demonstrated as a cell-surface marker for labeling and isolating CD8+ T cells with high reactivity to tumor-specific antigens (TSAs) from the tumor. Mechanistically, α2,6-sialyltransferase ST6Gal-I is transcriptionally suppressed upon the activation of intratumoral CD8+ T cells with high-avidity T cell receptors towards tumor antigens, which downregulates α2,6-sialylation and exposes galectin-1 ligands. Functionally, increased galectin-1 binding to TSA-reactive CD8+ T cells promotes T cell apoptosis and cancer immune evasion. This work underscores the functional significance and therapeutic promise of specific sialoglycans on TIICs.","dates":{"publication":"2026/08/01"},"accession":"GSE316540","cross_references":{"GSM":["GSM9455546","GSM9455554","GSM9455549","GSM9455548","GSM9455547","GSM9455553","GSM9455552","GSM9455551","GSM9455550"],"GPL":["24247"],"GSE":["316540"],"taxon":["Mus musculus"]}}