{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316637/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Arabidopsis thaliana"],"gds_type":["Non-coding RNA profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316637"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Immunization by mobile virus-derived siRNAs is crucial for plant antiviral silencing","description":"The movement of virus-derived (v)siRNAs between plant cells has prompted the widely held assumption that they help immunize as-yet-uninfected tissues. Ascertaining this role is challenging because it requires uncoupling vsiRNA mobility from viral movement while preserving viral virulence. Consequently, the contribution of non-cell-autonomous RNAi to plant antiviral immunity remains essentially unknown. Here, we investigated the naturally phloem-restricted infection of turnip yellows virus (TuYV) in the stereotypical Arabidopsis root system at cellular resolution. We uncovered intertwined, complex mechanisms operating in both vsiRNA-emitting and -recipient cells, that drive reiterated short-range (~3-4 cell) immunization events. We found that, while intracellular antiviral RNAi is nullified by TuYV, non-cell-autonomous immunization accounts mainly —if not exclusively— for the containment of viral tissue invasion, titers and symptoms severity at the whole-plant scale. Mobile vsiRNA-mediated immunization likewise spatially confines a TuYV unrelated virus over ~3-4 cells, uncovering a generic, essential, yet previously unexplored component of plant antiviral defence.","dates":{"publication":"2026/07/17"},"accession":"GSE316637","cross_references":{"GSM":["GSM9629000","GSM9457255","GSM9457256","GSM9628999","GSM9457257","GSM9457254"],"GPL":["34608","26208"],"GSE":["316637"],"taxon":["Arabidopsis thaliana"]}}