{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317446/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Drosophila melanogaster"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317446"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional regulation of abd-A and Abd-B in Drosophila larval adipocytes [CUT&Run]","description":"The Bithorax complex (BX-C) homeobox proteins specify segmental identities along the anterior-posterior axis during Drosophila embryogenesis. We previously showed that heterogeneous expression of the BX-C genes abd-A and Abd-B defines regional differences between thoracic and abdominal adipocytes. However, the mechanisms underlying this heterogeneity in adipocytes remain unclear. Here, we identify cis-regulatory regions and transcription factors that control abd-A and Abd-B expression specifically in abdominal, but not thoracic, adipocytes of the larval fat body. Using a promoter bashing approach, we identified a 627-bp fragment within the Abd-B locus and a ~6-kb region within the abd-A locus that are sufficient to drive heterogeneous expression in larval adipocytes. Yeast one-hybrid screening combined with functional analysis identified the transcription factors Lola (Longitudinals lacking), Lolal (Lola like), and Combgap/Cg as key regulators of Abd-B expression, with Prg Piragua/Prg and Svp (Seven up) acting as transcriptional activators. Furthermore, Lolal negatively regulates the expression of both Abd-B and abd-A in abdominal adipocytes. Together, these findings suggest that the heterogeneous expression of abd-A and Abd-B in the larval fat body is actively regulated by multiple transcription factors rather than passively maintained.","dates":{"publication":"2026/07/09"},"accession":"GSE317446","cross_references":{"GSM":["GSM9472399","GSM9472400","GSM9472397","GSM9472398","GSM9472395","GSM9472396"],"GPL":["25244"],"GSE":["317446"],"taxon":["Drosophila melanogaster"],"PMID":["[42244700]"]}}