GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317446/primaryOK200GenomicsDrosophila melanogasterGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317446GEOGSEfalseTranscriptional regulation of abd-A and Abd-B in Drosophila larval adipocytes [CUT&Run]The Bithorax complex (BX-C) homeobox proteins specify segmental identities along the anterior-posterior axis during Drosophila embryogenesis. We previously showed that heterogeneous expression of the BX-C genes abd-A and Abd-B defines regional differences between thoracic and abdominal adipocytes. However, the mechanisms underlying this heterogeneity in adipocytes remain unclear. Here, we identify cis-regulatory regions and transcription factors that control abd-A and Abd-B expression specifically in abdominal, but not thoracic, adipocytes of the larval fat body. Using a promoter bashing approach, we identified a 627-bp fragment within the Abd-B locus and a ~6-kb region within the abd-A locus that are sufficient to drive heterogeneous expression in larval adipocytes. Yeast one-hybrid screening combined with functional analysis identified the transcription factors Lola (Longitudinals lacking), Lolal (Lola like), and Combgap/Cg as key regulators of Abd-B expression, with Prg Piragua/Prg and Svp (Seven up) acting as transcriptional activators. Furthermore, Lolal negatively regulates the expression of both Abd-B and abd-A in abdominal adipocytes. Together, these findings suggest that the heterogeneous expression of abd-A and Abd-B in the larval fat body is actively regulated by multiple transcription factors rather than passively maintained.2026/07/09GSE317446GSM9472399GSM9472400GSM9472397GSM9472398GSM9472395GSM947239625244317446Drosophila melanogaster[42244700]