{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317860/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317860"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA-sequencing of myeloid cells in E0771 breast tumours from CD1d-KO and WT mice","description":"Myeloid cells play crucial roles in cancer progression, influencing tumour growth, metastasis, and response to immunotherapy. Tumour-infiltrating myeloid cells are highly heterogeneous, with functions ranging from anti-tumour to tumour-promoting. The mechanisms shaping these populations remain poorly understood and may offer therapeutic opportunities. Here, we identified the lipid-presenting molecule CD1d as a regulator of tumour progression and myeloid heterogeneity in the tumour microenvironment. CD1d-deficient mice exhibit reduced tumour growth and altered immune-infiltrates in breast cancer models. Similarly, targeting CD1d with an antibody, suppresses tumour growth, reshapes the myeloid landscape, and improves anti-PD-1 immunotherapy efficacy. The CD1d-dependent control of myeloid cell functional differentiation is cell-intrinsic and conserved in human and mouse. Single-cell RNA-sequencing revealed an enhanced proinflammatory program and accumulation of inflammatory monocytes in tumours from CD1d-KO animals. Subsequently, we generated a CD1d-KO-associated gene signature that predicts clinical outcomes and response to immunotherapy in breast cancer patients. Thus, CD1d could be targeted to alter tumour-infiltrating myeloid populations and enhance immunotherapy responses.","dates":{"publication":"2026/07/16"},"accession":"GSE317860","cross_references":{"GSM":["GSM9480139","GSM9480138","GSM9480137","GSM9480136","GSM9480135","GSM9480134"],"GPL":["34290"],"GSE":["317860"],"taxon":["Mus musculus"],"PMID":["[42593403]"]}}