{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317867/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317867"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Sleep Homeostasis Ensembles in the Hypothalamic PVN Reprogram Systemic Fuel Utilization via Adipose Lipolysis","description":"While sleep homeostasis is fundamental, its causal contribution on systemic metabolism remains incompletely understood. By decoupling sleep homeostasis from circadian influences, this study identifies a previously unrecognized neural mechanism linking compensatory sleep to whole-body metabolism. Using a minimally confounded sleep homeostasis model, spatial transcriptomics revealed the paraventricular nucleus of the hypothalamus (PVN) as the most transcriptionally active region during rebound sleep. TRAP-tagging and functional manipulations demonstrated that PVN sleep-homeostasis ensemble neurons promote NREM sleep while simultaneously shifting whole-body energy utilization toward lipid oxidation. Activation of these neurons reduced respiratory exchange ratio, downregulated systemic glucose metabolism, and enhanced sympathetic-driven lipolysis in white adipose tissue (WAT), particularly inguinal depot. Stable isotope tracing confirmed suppressed glucose metabolic flux in WAT during recovery sleep. These findings uncover a neural substrate through which sleep homeostasis regulates peripheral energy balance, revealing a metabolic trade-off that may underlie the increased diabetes risk associated with sleep disruption.","dates":{"publication":"2026/07/01"},"accession":"GSE317867","cross_references":{"GSM":["GSM9480340","GSM9480333","GSM9480344","GSM9480343","GSM9480332","GSM9480331","GSM9480342","GSM9480330","GSM9480341","GSM9480337","GSM9480336","GSM9480347","GSM9480335","GSM9480346","GSM9480334","GSM9480345","GSM9480339","GSM9480338"],"GPL":["34290"],"GSE":["317867"],"taxon":["Mus musculus"]}}