{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317890/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317890"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of interferon responses induced by IVT sgRNAs in CRISPRi HEK293T cells","description":"In this study, we investigated the transcriptional responses induced by in vitro–transcribed (IVT) sgRNAs in a CRISPR interference (CRISPRi) system. A total of 906 sgRNAs were generated using a commercially available IVT workflow and delivered to HEK293T cells stably expressing dCas9-KRAB-MECP2. RNA sequencing was performed to profile genome-wide transcriptional changes following sgRNA transfection. Compared with mock-transfected controls, sgRNA-transfected cells exhibited a strong and consistent upregulation of interferon-stimulated genes (ISGs), with prominent enrichment of interferon-α response pathways across the majority of sgRNAs. While most sgRNAs induced robust interferon signaling, a small subset showed a more limited transcriptional response. IFIT1 and IFIT2, two canonical markers of interferon activation, were consistently upregulated and were selected as representative genes for downstream validation. This dataset provides a systematic resource for assessing innate immune activation triggered by IVT sgRNAs in CRISPRi-based perturbation experiments.","dates":{"publication":"2026/06/01"},"accession":"GSE317890","cross_references":{"GSM":["GSM9481133","GSM9481012","GSM9481134","GSM9481013","GSM9481014","GSM9481135","GSM9481136","GSM9481015","GSM9480960","GSM9481130","GSM9481131","GSM9481010","GSM9481132","GSM9481011","GSM9480953","GSM9481009","GSM9480832","GSM9480831","GSM9480952","GSM9480951","GSM9480830","GSM9480950","GSM9480957","GSM9481126","GSM9481005","GSM9480836","GSM9481006","GSM9480956","GSM9481127","GSM9480835","GSM9480834","GSM9481128","GSM9481007","GSM9480955","GSM9480954","GSM9481129","GSM9480833","GSM9481008","GSM9480839","GSM9480959","GSM9480838","GSM9480837","GSM9480958","GSM9481122","GSM9481001","GSM9481123","GSM9481002","GSM9481003","GSM9481124","GSM9481125","GSM9481004","GSM9481120","GSM9481000","GSM9481121","GSM9480942","GSM9480821","GSM9481119","GSM9480941","GSM9480820","GSM9480940","GSM9480825","GSM9480946","GSM9481115","GSM9480945","GSM9480824","GSM9481116","GSM9481117","GSM9480944","GSM9480823","GSM9480943","GSM9480822","GSM9481118","GSM9480829","GSM9480828","GSM9480949","GSM9480948","GSM9480827","GSM9480947","GSM9480826","GSM9481111","GSM9481112","GSM9481113","GSM9481114","GSM9481110","GSM9480931","GSM9481108","GSM9480810","GSM9481109","GSM9480930","GSM9480814","GSM9481104","GSM9480935","GSM9480934","GSM9480813","GSM9481105","GSM9481106","GSM9480933","GSM9480812","GSM9480811","GSM9481107","GSM9480932","GSM9480939","GSM9480818","GSM9480817","GSM9480938","GSM9480937","GSM9480816","GSM9480936","GSM9480815","GSM9480819","GSM9481100","GSM9481101","GSM9481102","GSM9481103","GSM9480920","GSM9480924","GSM9480923","GSM9480922","GSM9480921","GSM9480928","GSM9480927","GSM9480926","GSM9480925","GSM9480929","GSM9481050","GSM9481051","GSM9481056","GSM9481057","GSM9481058","GSM9481059","GSM9480883","GSM9481052","GSM9480882","GSM9481053","GSM9481054","GSM9480881","GSM9480880","GSM9481055","GSM9480997","GSM9480876","GSM9480996","GSM9480875","GSM9480874","GSM9480995","GSM9480994","GSM9480873","GSM9481049","GSM9480879","GSM9480999","GSM9480878","GSM9480877","GSM9480998","GSM9481160","GSM9481040","GSM9481161","GSM9481166","GSM9481045","GSM9481046","GSM9481167","GSM9481168","GSM9481047","GSM9481169","GSM9481048","GSM9481162","GSM9480993","GSM9481041","GSM9480872","GSM9481163","GSM9480871","GSM9481042","GSM9480992","GSM9481043","GSM9480991","GSM9480870","GSM9481164","GSM9481165","GSM9481044","GSM9480990","GSM9480986","GSM9480865","GSM9480985","GSM9480864","GSM9480984","GSM9480863","GSM9480983","GSM9480862","GSM9481159","GSM9480869","GSM9481038","GSM9480868","GSM9481039","GSM9480989","GSM9480988","GSM9480867","GSM9480987","GSM9480866","GSM9481150","GSM9481034","GSM9481155","GSM9481156","GSM9481035","GSM9481157","GSM9481036","GSM9481037","GSM9481158","GSM9480982","GSM9481151","GSM9481030","GSM9480861","GSM9481152","GSM9480860","GSM9481031","GSM9480981","GSM9480980","GSM9481153","GSM9481032","GSM9481154","GSM9481033","GSM9480854","GSM9480975","GSM9480974","GSM9480853","GSM9480973","GSM9480852","GSM9480851","GSM9480972","GSM9480979","GSM9481148","GSM9481027","GSM9480858","GSM9480857","GSM9481149","GSM9481028","GSM9480978","GSM9480977","GSM9480856","GSM9481029","GSM9480976","GSM9480855","GSM9480859","GSM9481023","GSM9481144","GSM9481145","GSM9481024","GSM9481146","GSM9481025","GSM9481026","GSM9481147","GSM9480971","GSM9481140","GSM9480850","GSM9481020","GSM9480970","GSM9481141","GSM9481142","GSM9481021","GSM9481143","GSM9481022","GSM9480964","GSM9480843","GSM9480963","GSM9480842","GSM9480962","GSM9480841","GSM9480840","GSM9480961","GSM9481137","GSM9480968","GSM9480847","GSM9481016","GSM9481017","GSM9480967","GSM9481138","GSM9480846","GSM9480845","GSM9481139","GSM9480966","GSM9481018","GSM9481019","GSM9480965","GSM9480844","GSM9480849","GSM9480848","GSM9480969","GSM9481092","GSM9481093","GSM9481094","GSM9481095","GSM9481090","GSM9481091","GSM9481096","GSM9481097","GSM9481098","GSM9481099","GSM9481081","GSM9481082","GSM9481083","GSM9481084","GSM9481080","GSM9481089","GSM9481085","GSM9481086","GSM9481087","GSM9481088","GSM9481070","GSM9481071","GSM9481072","GSM9481073","GSM9481078","GSM9481079","GSM9481074","GSM9481075","GSM9481076","GSM9481077","GSM9480898","GSM9480897","GSM9480896","GSM9480895","GSM9480899","GSM9481060","GSM9481061","GSM9481062","GSM9481067","GSM9480890","GSM9481068","GSM9481069","GSM9480894","GSM9481063","GSM9480893","GSM9481064","GSM9481065","GSM9480892","GSM9480891","GSM9481066","GSM9480887","GSM9480886","GSM9480885","GSM9480884","GSM9480889","GSM9480888","GSM9480913","GSM9480912","GSM9480911","GSM9480910","GSM9480917","GSM9480916","GSM9480915","GSM9480914","GSM9480919","GSM9480918","GSM9480902","GSM9480901","GSM9480900","GSM9480906","GSM9480905","GSM9480904","GSM9480903","GSM9480909","GSM9480908","GSM9480907"],"GPL":["24676"],"GSE":["317890"],"taxon":["Homo sapiens"]}}