GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317890/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317890GEOGSEfalseTranscriptomic profiling of interferon responses induced by IVT sgRNAs in CRISPRi HEK293T cellsIn this study, we investigated the transcriptional responses induced by in vitro–transcribed (IVT) sgRNAs in a CRISPR interference (CRISPRi) system. A total of 906 sgRNAs were generated using a commercially available IVT workflow and delivered to HEK293T cells stably expressing dCas9-KRAB-MECP2. RNA sequencing was performed to profile genome-wide transcriptional changes following sgRNA transfection. Compared with mock-transfected controls, sgRNA-transfected cells exhibited a strong and consistent upregulation of interferon-stimulated genes (ISGs), with prominent enrichment of interferon-α response pathways across the majority of sgRNAs. While most sgRNAs induced robust interferon signaling, a small subset showed a more limited transcriptional response. IFIT1 and IFIT2, two canonical markers of interferon activation, were consistently upregulated and were selected as representative genes for downstream validation. This dataset provides a systematic resource for assessing innate immune activation triggered by IVT sgRNAs in CRISPRi-based perturbation experiments.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 sapiens