{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317913/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317913"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"DADA inhibits tumor growth by enhancing CD8+ T cell stemness","description":"To elucidate the mechanism by which DADA enhances the CD8+ T cell anti-tumor immune responses, we performed signal-cell RNA-sequencing (scRNA-seq) on tumor-infiltrating CD45+ T cells isolated from ctrl- or DADA-treated MC38-bearing mice.In this study, we found that diisopropylamine dichloroacetate (DADA) enhances CD8⁺ T cell-mediated anti-tumor immunity and promotes the accumulation of Tpex cells in the tumor microenvironment. Mechanistically, DADA promotes the conversion of pyruvate to acetyl-CoA by inhibiting pyruvate dehydrogenase kinase, thereby increasing oxidative phosphorylation (OXPHOS) levels and mitochondrial fitness, and ultimately enhancing the stemness of CD8⁺ T cells. In mouse models, DADA treatment significantly improves the efficacy of PD-1 blockade. Furthermore, the addition of DADA to the in vitro expansion system of chimeric antigen receptor (CAR)-T cells confers stemness characteristics to the cells, thereby enhancing their anti-tumor efficacy.","dates":{"publication":"2026/05/20"},"accession":"GSE317913","cross_references":{"GSM":["GSM9481876","GSM9481877"],"GPL":["24247"],"GSE":["317913"],"taxon":["Mus musculus"],"PMID":["[41758776]"]}}