{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318012/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318012"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing of E18.5 mouse hindstomach epithelium and bulk RNA sequencing of its derived 3D organoids across distinct YAP activity states [RNA-seq for differentiation]","description":"Hippo signaling plays a crucial role in the development of multiple organs. This study consists of two transcriptomic datasets.Single-cell RNA sequencing was conducted on E18.5 mouse hindstomach epithelial cells of littermate control, YAP5SA-overexpressing and Yap knockout mice, where YAP5SA represents a constitutively active form of Yap. Bulk RNA sequencing was performed to analyze transcriptional changes in 3D organoids derived from the glandular embryonic hindstomach epithelium of control and YAP5SA-overexpressing mice. Among these, organoids were cultured under two conditions: growth medium for 5 days, or growth medium for 5 days followed by differentiation medium for an additional 7 days. Comparative gene expression profiling provides valuable insights into the role of the Hippo signaling pathway in regulating diverse biological processes during glandular stomach epithelial development.","dates":{"publication":"2026/05/28"},"accession":"GSE318012","cross_references":{"GSM":["GSM9484492","GSM9484491","GSM9484490","GSM9484489","GSM9484488","GSM9484485","GSM9484487","GSM9484486"],"GPL":["24247"],"GSE":["318012"],"taxon":["Mus musculus"]}}